About The Cytometry Toolkit
The Cytometry Toolkit comprises a number of different calculators that aid in setting up and running cytometers, including sorting flow cytometers. Features: *Resolution calculator: Calculates the S or Separation parameter
The Cytometry Toolkit comprises a number of different calculators that aid in setting up and running cytometers, including sorting flow cytometers.
Features:
*Resolution calculator:
Calculates the S or Separation parameter of two dim populations. Useful if you want a quickly test a new optical filter or a new dye in your assay.
*Q B and S calculator:
This calculator is like the Resolution Calculator by gives much more information such as the Quantum efficiency of the system, the Background and a calculated separation parameter. It also includes the Separation Parameter as reported by the Resolution calculator.
*Simple Lens Calculator:
Is used only for thin spherical lenses. This calculator calculates; the Laser Spot Size at the focus, the Wavelength, the Focal Length or the laser beam’s Diameter at the back of the focusing lens.
*Two Recovery Calculators:
Rmax# and Rmax %. These calculators calculate the recovery from a sort. One simply needs to sample the waste stream to assess the efficiency of the sort. Really useful in assessing if the Break-off Point is optimal and is a non-invasive way of calculating the recovery from rare population sorts without having to sample and count the rare particles.
*Poisson sort Calculator
An extension of the Recovery calculators though usable on it’s own, it will calculate the expected recoveries you should get back after a sort if your sort sample behaves in a Poisson way, that is, your particles in the sample are mono-dispersed. The calculator also reports the sort time.
Feedback/suggestions are always welcome. I will update this periodically with bug fixes and new features for at least two years. I hope you find this useful…
Previous Versions
Here you can find the changelog of The Cytometry Toolkit since it was posted on our website on 2016-12-25 22:27:06.
The latest version is 1.2.1 and it was updated on 2024-04-19 18:44:09. See below the changes in each version.
The Cytometry Toolkit version ToolKit release 1.2.1
Updated At: 2012-12-31
Changes: . Entry for “Non-Target” changed to “Total” in the Rmax# calculation
. The lens calculation now calculates the Depth of Focus (Rayleigh range)
. Changes reflected in the help menus
. Added Rmax# error handler when inputs are incorrect
. General Corrections in the help menus
Version 1.2.1
. The lens calculator sometimes gave incorrect Depth of Focus now Fixed.
. Fix for Rmax# calculator nows shows the "To Poisson" button after calculation.
Disclaimer
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